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US 2019/0314397 A1Published application
Methods For Directed Folding Assembly Or Dimerization Of Proteins By Templated Assembly Reactions
AssigneeTriBiotica LLC
InventorsIan Dunn, Matthew Lawler
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Abstract
The present disclosure provides nucleic acid molecules, compositions, and kits comprising the same, and methods for producing templated assembly products.
Claims15
A haplomer-ligand complex comprising:
- a haplomer, wherein the haplomer comprises a polynucleotide that is substantially complementary to a target nucleic acid molecule;
- and
- a ligand linked to the 5′ or 3′ terminus of the haplomer, wherein the ligand comprises a ligand partner binding site.
A bottle haplomer-ligand complex comprising:
- a) a bottle haplomer, wherein the bottle haplomer comprises a polynucleotide, wherein the polynucleotide comprises:
- i) a first stem portion comprising from about 10 to about 20 nucleotide bases;
- ii) an anti-target loop portion comprising from about 16 to about 40 nucleotide bases and having a first end to which the first stem portion is linked, wherein the anti-target loop portion is substantially complementary to a target nucleic acid molecule;
- and
- iii) a second stem portion comprising from about 10 to about 20 nucleotide bases linked to a second end of the anti-target loop portion, wherein the first stem portion is substantially complementary to the second stem portion;
- and
- b) a ligand linked to the terminal end of either the first stem portion or the second stem portion, wherein the ligand comprises a ligand partner binding site;
- wherein the Tm of the anti-target loop portion:target nucleic acid molecule is greater than the Tm of the first stem portion:second stem portion.
5 more elements
A compound having the formula:
- where m is from 3 to 6;
- having the formula:
- where m is from 3 to 6;
- having the formula;
- where n is from 1 to 6;
- having the formula:
- where x is from 1 to 6;
- having the formula:
- where x is from 1 to 6;
- or having the formula:
7 more elements
A fusion protein comprising a fragment of a protein of interest fused to a ligand binding domain, wherein the ligand binding domain is an FKBP domain or an FRB domain.
Dependencies are inferred from the claim text.