Metabolically competent cells, methods of making, and uses thereof
AssigneesUniversity of Florida Research Foundation, Inc., The Research Foundation for The State University of New York
InventorsCHRISTOPHER DILLON VULPE, AMIN SOBH, DAVID MICHAEL FAULKNER, ABDERRAHMANE TAGMOUNT, MICHAEL FASULLO
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Abstract
Provided herein are genetically engineered cells containing one or more modulated metabolic genes, where the expression of the modulated metabolic gene(s) can be greater than that of an unmodified control. Also provided herein are methods of making the genetically engineered cells using synergistic activation mediator CRISPR-Cas9. Further provided herein are high throughput assays that can employ the genetically engineered cells provided herein.
Claims21
A genetically engineered cell comprising:
- one or more modulated metabolic genes, wherein the expression of the one or more modulated metabolic genes is greater than the expression in an unmodified control cell.
-15. (canceled)
A screening assay comprising the steps of:
- exposing a genetically engineered cell with a test chemical or compound for a period of time, wherein the genetically engineered cell is comprised of one or more modulated metabolic genes, wherein the expression of the one or more modulated metabolic genes is greater than the expression in an unmodified control cell;
- and
- measuring a physiologic characteristic of the genetically engineered cell.
Dependencies are inferred from the claim text.