Apparatus for determining cellular composition information in one or more tissue samples

An apparatus for determining cellular composition information in one or more tissue sample microscopic images, comprising:

• an input unit;
• and
• a processing unit;
• wherein, the input unit is configured to provide the processing unit with at least one 2D image of at least one tissue sample, wherein first image data of the at least one 2D image relates to a non-specific staining of a tissue sample of the at least one tissue sample, and wherein second image data of the at least one 2D image relates to specific staining of a tissue sample of the at least one tissue sample configured to target a first subtype of cell;
• wherein (a) the tissue sample that has undergone specific staining is the same as the tissue sample that has undergone non-specific staining, or (b) the tissue sample that has undergone specific staining is different to the tissue sample that has undergone non-specific staining;
• wherein, the processing unit is configured to determine a non-specific cellular composition cell density map on the basis of a morphological feature analysis of the first image data;
• wherein, the processing unit is configured to determine a specific cellular composition cell density map on the basis of a stain intensity distribution analysis of the second image data;
• wherein, the processing unit is configured to determine information regarding cellular composition in the at least one tissue sample on the basis of the non-specific cell density map and the specific cell density map, wherein the information regarding the cellular composition comprises at least one quantification of the first subtype of cell, and comprises at least one quantification of a second subtype of cell, wherein the specific staining is configured to target at least one subtype of cell that is different to the second subtype of cell;
• and wherein if a difference between the non-specific cell density map and the specific cell density map is positive, then an indication is made that there are unaccounted infiltrate cells.