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US 2019/0293623 A1Published application
Planar High-Speed Molecule Sequencer
AssigneeWisconsin Alumni Research Foundation
InventorsRobert H. Blick, Abhishek Bhat, Paul Gwozdz
Life of this patent
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Abstract
A molecule sequencer uses a planar nanochannel for aligning molecules to flow past impedance interrogation electrodes mounted across the channel for rapid sequencing. The electrodes may be fixed to the channel walls to provide improved registration by co-fabrication with the channel. Extended channel length over normally used nanopores can provide improved control of longer molecules and allow parallel sequencers to be readily fabricated on a single substrate.
Claims20
A molecule sequencing system comprising:
- a substrate extending along a plane and supporting at least one analysis cell comprising:
- (a) a nanometer-width nanochannel in the substrate extending along a plane between an entrance point and exit point of the nanochannel;
- (b) a first and second conductive electrode each fixed relative to the nanochannel in flanking opposition across a width of the nanochannel between the entrance and exit point of the nanochannel;
- and
- measurement circuitry applying a radiofrequency signal across the first and second conductive electrodes to measure a change in the radiofrequency signal with a passage of a molecule through the nanochannel between the first and second conductive electrodes.
2 more elements
A method of sequencing a molecule sequencing system using
- a substrate extending along a plane and supporting at least one analysis cell comprising:
- (a) a nanometer-width nanochannel in the substrate extending along a plane between an entrance and exit point of the nanochannel;
- (b) a first and second conductive electrode each fixed relative to the nanochannel in flanking opposition across a width of the nanochannel between the entrance and exit point of the nanochannel;
- and
- measurement circuitry applying a radiofrequency signal across the first and second conductive electrodes to measure a change in the radiofrequency signal with a passage of a molecule through the nanochannel between the first and second conductive electrodes, the method comprising:
- (a) pumping a liquid through the nanochannel containing molecules to be analyzed such that the nanochannels cause the molecules to axially align with the channels;
- (b) during motion of the molecules between the first and second conductive electrodes, obtaining multiple measurements of change in radiofrequency signal;
- and
- (c) correlating the changes in radiofrequency signal to molecule components.
6 more elements
Dependencies are inferred from the claim text.