Apparatus for optically exciting and detecting fluorescence

Abstract

Apparatus for optically exciting fluorescence and detecting light is disclosed. The apparatus comprises a device for optically exciting fluorescence. The device comprises a transparent substrate having first and second opposite faces and a multilayer stack disposed on the second face of the substrate. The multilayer stack comprises a first layer having first and second opposite faces and a first refractive index and a second layer having first and second opposite faces and a second refractive index. The first face of the first layer is disposed on the second face of the substrate. The first face of the second layer is disposed on the second face of the first layer such that the first layer is interposed between the second layer and the substrate. The substrate has a third refractive index. The first refractive index is less than the second refractive index and the third refractive index. The device further comprises a light source carried by the first face of the substrate and arranged to emit light towards the first face of the first layer. The apparatus comprises a detector directed at the substrate, the substrate interposed between the waveguide and the detector for detecting fluorescence.

Dependencies are inferred from the claim text.