Modified nucleoside phosphates with high thermal stability
AssigneeRoche Molecular Systems, Inc.
InventorAlexander Nierth
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Abstract
The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
Claims17
A method of detecting the presence or absence of a target nucleic acid sequence in a sample comprising:
- a) performing an amplifying step comprising contacting the sample with amplification reagents to produce an amplification product if the target nucleic acid sequence is present in the sample, and
- b) detecting the amplification product,
- wherein the amplification reagents comprise a modified nucleoside polyphosphate having a structure of:
- where R=purine or pyrimidine base or an analog, and n=1-4.
A method of amplifying a target nucleic acid sequence using amplification reagents wherein the amplification reagents comprise a nucleoside polyphosphate having a structure of:
- where R=purine or pyrimidine base or an analog, and n=1-4.
A kit for amplification of a target nucleic acid sequence comprising a nucleic acid polymerase, buffer and a nucleoside polyphosphate having a structure of:
- where R=purine or pyrimidine base or an analog, and n=1-4.
Dependencies are inferred from the claim text.