Method for Determining the Number of Sites of Infection of a Cell Culture
AssigneePerkinElmer Cellular Technologies Germany GmbH
InventorNorbert Garbow
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Abstract
Provided herein is a method for determining the number of sites of infection of a cell culture, including the steps of: infecting a cell culture arranged in a sample carrier with viruses, counting any infected areas of the cell culture by means of a transmitted light method, marking infected cells with fluorescence markers, counting infected areas of the cell culture by means of a fluorescence analysis method, and evaluating area by area the areas determined in both methods for determining the number of sites of infection.
Claims18
A method for determining the number of sites of infection of a cell culture, comprising the steps of:
- infecting a cell culture arranged in a sample carrier with viruses;
- counting any infected areas of the cell culture by means of a transmitted light method;
- marking infected cells with fluorescence markers;
- counting infected areas of the cell culture by means of a fluorescence analysis method;
- and
- evaluating, area by area, the areas determined in both the transmitted light and fluorescence analysis for determining the number of sites of infection.
Dependencies are inferred from the claim text.