Recombinant yeast for producing 2,3-butanediol including pyruvate decarboxylase derived from candida tropicolis and method for producing 2,3-butanediol using the same

Abstract

Disclosed are a recombinant yeast for producing 2,3-butanediol and a method for producing 2,3-butanediol using the same. By introducing Candida tropicalis-derived Pdc, which is less active than its own pyruvate decarboxylase (Pdc), into the cells of the strain, the recombinant yeast can synthesize acetyl-CoA, while avoiding production of ethanol, thereby increasing the strain growth rate and the substrate consumption rate and ultimately greatly improving productivity of 2,3-butanediol. Disclosed is a method for producing 2,3-butanediol. Conventional methods for producing 2,3-butanediol using Saccharomyces cerevisiae (yeast) inevitably cause production of a great amount of glycerol as a by-product, in addition to production of 2,3-butanediol. However, the yeast strain according to the present invention can produce 2,3-butanediol with high purity, high yield and high productivity, while inhibiting production of glycerol.

Dependencies are inferred from the claim text.