Producing recombinant products in contained systems
AssigneeImpossible Foods Inc.
InventorsSimon Christopher Davis, Patrick O'Reilly Brown, Michael Lassner
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Abstract
This document relates to methods of making recombinant target products in transgenic plants in a contained system, and more particularly to separating the production of plant biomass from the production of the target product by selectively expressing the target product during germination in a contained system.
Claims29
A method of recombinantly producing a member of the globin family in a contained system, the method comprising:
- a) germinating a plurality of transgenic seeds in the contained system to produce a plurality of seedlings or plants, wherein each transgenic seed comprises a nucleic acid construct, the nucleic acid construct comprising an inducible promoter operably linked to (i) a nucleic acid encoding the member of the globin family or (ii) a nucleic acid encoding a polypeptide in a biosynthesis pathway of heme;
- b) contacting the seedlings or plants with an inducer for the inducible promoter;
- and
- c) isolating the member of the globin family from the plurality of seedlings or plants.
A method of recombinantly producing a member of the globin family in a contained system, the method comprising:
- a) germinating a plurality of transgenic seeds in the contained system in the presence of an inducer to produce a plurality of seedlings, wherein each transgenic seed comprises a nucleic acid construct, the nucleic acid construct comprising an inducible promoter operably linked to (i) a nucleic acid encoding the member of the globin family or (ii) a nucleic acid encoding a polypeptide in a biosynthesis pathway of heme;
- and
- b) isolating the target product from the plurality of seedlings.
A method of recombinantly producing a member of the globin family in a contained system, the method comprising:
- a) germinating a plurality of transgenic seeds in the contained system to produce a plurality of seedlings, wherein each transgenic seed comprises a nucleic acid construct, the nucleic acid construct comprising a germination specific promoter operably linked to (i) a nucleic acid encoding the member of the globin family or (ii) a nucleic acid encoding a polypeptide in a biosynthesis pathway of heme;
- and
- b) isolating the target product from the plurality of seedlings.
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Dependencies are inferred from the claim text.