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US 2019/0292553 A1Published application
A method for base editing in plants
AssigneeInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
InventorsCaixia GAO, Yuan ZONG
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Abstract
The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.
Claims23
A system for performing base editing to a target sequence in a plant genome, comprising at least one of the following (i) to (v):
- i) a base editing fusion protein, and a guide RNA;
- ii) an expression construct comprising a nucleotide sequence encoding a base editing fusion protein, and a guide RNA;
- iii) a base editing fusion protein, and an expression construct comprising a nucleotide sequence encoding a guide RNA;
- iv) an expression construct comprising a nucleotide sequence encoding a base editing fusion protein, and an expression construct comprising a nucleotide sequence encoding a guide RNA;
- v) an expression construct comprising a nucleotide sequence encoding base editing fusion protein and a nucleotide sequence encoding guide RNA;
- wherein said base editing fusion protein contains a nuclease-inactivated Cas9 domain and a deaminase domain, said guide RNA can target said base editing fusion protein to the target sequence in the plant genome.
3 more elements
Dependencies are inferred from the claim text.