Automated cell processing methods, modules, instruments, and systems

An automated stand-alone multi-module cell editing instrument for performing inducible and recursive nuclease editing comprising:

• a receptacle configured to receive cells;
• a receptacle configured to receive nucleic acids;
• a growth module comprising at least one rotating growth vial configured to grow the cells;
• a filtration module configured to concentrate the cells and/or render the cells electrocompetent;
• a transformation module configured to introduce the nucleic acids into the cells;
• a nuclease-directed editing module configured to select for transformed cells, activate the inducible nuclease editing system, and allow the introduced nucleic acids to edit nucleic acids in the cells to produce edited cells;
• a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a script;
• and
• an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the filtration module, from the filtration module to the transformation module, from the nucleic acid receptacle to the transformation module, from the transformation module to the nuclease-directed editing module, and from the nuclease-directed editing module to the growth module, all without user intervention.