Nucleic acids encoding chimeric polypeptides for library screening
AssigneeRoche Diagnostics Operations, Inc.
InventorsDavid Casagolda Vallribera, Frank Kroner, Michael Schraeml
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Abstract
The present disclosure is directed to a library of chimeric polypeptides comprising one or more fragments from the peptidyl-prolyl cis/trans isomerase or FKBP family displaying one or more polypeptides on one or more FKBP members and its use in methods for the screening and selection of constrained peptide surrogates exhibiting binding activity versus predetermined target molecules.
Claims15
A composition comprising nucleic acid molecules encoding a population of chimeric polypeptide species, wherein each nucleic acid molecule encodes a chimeric polypeptide species that comprises a polypeptide according to formula I
- NH2—S2—X—S1—COOH (formula I),
- wherein
- X is an amino acid sequence comprising a variable sequence,
- S2 and S1 are non-variable and non-overlapping amino acid sequences derived (i) from a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or (ii) from a polypeptide from the FKBP-fold domain family,
- between two amino acids denotes a peptide bond, and
- X is inserted in place of the insert-in-flap-domain (IF-domain) of the polypeptide of (i) or the polypeptide of (ii).
Dependencies are inferred from the claim text.