Engineered glucosyltransferases

A non-native glucosyltransferase comprising at least one amino acid substitution at a position corresponding with amino acid residue Leu-428, Ala-472, Leu-513, Met-529, Phe-607, Asn-613, Gln-616, Ser-631, Gly-633, Phe-634, Thr-635, or Phe-951 of SEQ ID NO:62,

• wherein the non-native glucosyltransferase synthesizes alpha-glucan comprising 1,3-linkages, and
• wherein the non-native glucosyltransferase has:
• (i) an alpha-glucan yield that is higher than the alpha-glucan yield of a second glucosyltransferase that only differs from the non-native glucosyltransferase at the substitution position(s), and/or
• (ii) a leucrose yield that is lower than the leucrose yield of the second glucosyltransferase;
• wherein the non-native glucosyltransferase comprises a catalytic domain that is at least about 90% identical to residues 55-960 of SEQ ID NO:4, residues 54-957 of SEQ ID NO:65, residues 55-960 of SEQ ID NO:30, residues 55-960 of SEQ ID NO:28, or residues 55-960 of SEQ ID NO:20.