Dual-Purpose Viral Transduction and Electroporation Device

A viral transduction and/or electroporation method, comprising:

• loading one side of a membrane with cells, viruses for transduction, and cargo for electroporation;
• flowing fluid through the membrane to colocalize the cells, the viruses and the cargo against the membrane;
• and
• establishing an electrical field across the membrane to electroporate cell pinned to the membrane.

A viral transduction and/or electroporation system, comprising:

• a membrane;
• electroporation electrodes;
• and
• an electrical voltage source for establishing an electrical field at the membrane and between the electrodes,
• means for tuning a distance between the electrodes and the membrane.

A viral transduction and/or electroporation system, comprising:

• a membrane separating two cavities;
• electroporation electrodes;
• an electrical voltage source for establishing an electrical field at the membrane and between the electrodes;
• an electroporation buffer reservoir for supplying electroporation buffer into the cavities;
• a viral transduction reservoir for supplying viruses into the cavities;
• and
• a cell media reservoir for supplying cell media into the cavities.

A viral transduction and/or electroporation system, comprising:

• a membrane separating two cavities;
• electroporation electrodes;
• an electrical voltage source for establishing an electrical field at the membrane and between the electrodes;
• a pinning pump for pinning particles against the membrane;
• and
• an unpinning pump for unpinning particles from the membrane.