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AssigneeRijksuniversiteit Groningen
InventorsGiovanni Maglia, Carsten Wloka, Natalie Lisa Mutter, Misha Soskine, Gang Huang
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The invention relates generally to the field of nanopores and the use thereof in various applications, such as analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through a nanopores. Provided is a system comprising a funnel-shaped proteinaceous nanopore comprising an a-helical pore-forming toxin that is a member from the actinoporin protein family, more in particular Fragaceatoxin C (FraC), a mutant FraC, a FraC paralog, or a FraC homolog.
A system comprising a funnel-shaped proteinaceous nanopore comprising an α-helical pore-forming toxin that is a member from the actinoporin protein family, wherein the α-helical pore-forming toxin is Fragaceatoxin C (FraC), a mutant FraC, a FraC paralog, or a FraC homolog.
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A mutant Fragaceatoxin C (FraC) nanopore comprising at least a first mutant FraC monomer comprising at least one substitution of a negatively charged amino acid residue in the narrow part of the pore and/or at least one substitution of a neutral amino acid residue in the narrow part of the pore into a positively charged amino acid residue, preferably comprising a substitution at position 3 or position 10, or both positions 3 and 10.
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Dependencies are inferred from the claim text.