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US 2019/0270986 A1Published application
Compositions and methods for activating silent gene clusters
AssigneeZymergen Inc.
InventorsOliver Liu, Jeffrey Hoon Kim
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Abstract
The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor.
Claims8
A method of identifying a product in a bacterial cell comprising:
- (a) infecting a bacterial cell with a bacteriophage, wherein the bacteriophage comprises a nucleotide sequence that encodes one or more bacterial transcription factors,
- (b) identifying products produced by the bacterial cell,
- (c) comparing the products identified in step (b) to the products produced from a control bacterial cell that has not been infected with a bacteriophage that comprises a nucleotide sequence that encodes the one or more bacterial transcription factors, and
- (d) identifying a product not produced by the control bacterial cell when there is a difference between the products identified in the bacterial cell and the control bacterial cell.
1 more element
Dependencies are inferred from the claim text.