Design, synthesis and use of synthetic nucleotides comprising charge mass tags
AssigneeQuantumdx Group Limited
InventorsJonathan J. O'Halloran, Joseph H. Hedley
Life of this patent
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Abstract
Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced.
Claims20
A reporter composition, comprising:
- a nucleotide or its derivative;
- a high charge mass moiety comprising an aromatic or aliphatic skeleton, comprising one or more charged groups selected from the group consisting of a tertiary amino group, a carboxyl group, a hydroxyl group, a phosphate group, a phenolic hydroxy group, any derivatives thereof, and any combinations thereof, wherein the one or more charged groups comprise a charge mass that is sufficient to generate a detectable change in a property of a sensitive detection nanostructure or nano-/micro-sensor operably coupled to the reporter composition;
- and
- a linker molecule attached to the nucleotide or its derivative and the high charge mass moiety, wherein the linker molecule photocleavable cleavable linker molecule comprises one or more selected from the group consisting of the following compounds 6-10, any derivatives thereof, and any combinations thereof:
A reporter composition, comprising:
- a nucleotide or its derivative;
- a high charge mass moiety comprising
- one or more selected from the group consisting of the following compounds 1-5, any derivatives thereof, and any combinations thereof:
- wherein the compounds comprise a charge mass that is sufficient to generate a detectable change in a property of a sensitive detection nanostructure or nano-/micro-sensor operably coupled to the reporter composition;
- and
- a linker molecule attached to the nucleotide or its derivative and the high charge mass moiety, wherein the linker molecule comprises a linear or branched chain comprising one or more selected from the group consisting of an alkyl group, an oxy alkyl group, an alcohol group, a carboxyl group, an amine group, an amide group, an aromatic group, and a naphthalene group, any derivatives thereof, and any combinations thereof.
The reporter composition of any of the previous claims, wherein the linker molecule or the high charge mass moiety is configured not to affect nucleotide polymerization by a polymerase.
The reporter composition of any of the previous claims, wherein the high charge mass moiety is configured to extend out from a nucleotide polymerase complex.
The reporter composition of any of the previous claims, wherein the linker molecule or the high charge mass moiety is configured to protrude out from a nascent chain so as to reach-down toward the sensitive detection nanostructure or sensor.
The reporter composition of any of the previous claims, wherein the linker molecule and/or the high charge mass moiety is configured to be removable.
The reporter composition of any of the previous claims, wherein the reporter composition comprises one or more selected from the group consisting of the following compounds, any derivatives thereof, and any combinations thereof:
Dependencies are inferred from the claim text.