Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof
AssigneeWayne State University
InventorZhuo-Hua Pan
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Abstract
The invention provides compositions and kits including at least one nucleic acid or polypeptide molecule encoding for a mutant CoChop protein. Methods of the invention include administering a composition comprising a mutant CoChop to a subject to preserve, improve, or restore phototransduction. Preferably, the compositions and methods of the invention are provided to a subject having impaired vision, thereby restoring vision to normal levels.
Claims24
An isolated light-activated ion channel polypeptide comprising the amino acid sequence of SEQ ID NO:2 comprising one or more amino acid modifications wherein the light-activated ion channel polypeptide has a least one of a greater level of an ion flux and a greater level of proton flux compared to the light-activated ion channel polypeptide of SEQ ID NO:2 when expressed in a cell membrane and contacted with activating light.
An isolated polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:
- 3-10.
An isolated polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:
- 3-10 comprising one or more amino acid modifications, wherein the one or more modification is a substitution, deletion or insertion.
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Dependencies are inferred from the claim text.