Insecticidal polypeptides having broad spectrum activity and uses thereof
AssigneePioneer Hi-Bred International, Inc.
InventorsAndre R Abad, Thomas Chad Wolfe, Lan Zhou
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Abstract
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims20
An isolated nucleic acid molecule operably linked to a heterologous regulatory element, wherein the isolated nucleic acid is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11 and SEQ ID NO:
- 13;
- (b) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14;
- and
- (c) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14, wherein the polypeptide has insecticidal activity.
An isolated polypeptide operably linked to a heterologous signal peptide, wherein the isolated polypeptide is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14;
- and
- (b) a polypeptide having at least 95% sequence identity to a polypeptide sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14, wherein the polypeptide has insecticidal activity.
(canceled)
(canceled)
A plant or plant cell having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule that encodes a polypeptide having pesticidal activity, wherein said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11 and SEQ ID NO:
- 13;
- (b) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14;
- and
- (c) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14, wherein the polypeptide has insecticidal activity;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
A method of producing a transgenic plant or plant cell resistant to a pest, comprising introducing into said plant or plant cell an expression vector comprising a nucleic acid molecule that encodes a pesticidal polypeptide, wherein said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11 and SEQ ID NO:
- 13;
- (b) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14;
- and
- (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14, wherein the polypeptide has insecticidal activity.
A method of protecting a plant from a pest, comprising introducing into said plant or plant cell an expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11 and SEQ ID NO:
- 13;
- (b) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14;
- and
- (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12 and SEQ ID NO:
- 14, wherein the polypeptide has insecticidal activity.
-28. (canceled)
Dependencies are inferred from the claim text.