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US 2019/0240595 A1Published application
Use of electrochemical devices or systems comprising redox-functionalized electrodes for bioseparation and biocatalysis
AssigneeMassachusetts Institute of Technology
InventorsXiao Su, Trevor Alan Hatton
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Abstract
Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more biomolecules from a solution, and methods of using the same. Methods for using the electrochemical devices for biocatalysis are also described herein.
Claims37
A method of separating a target biomolecule from a solution comprising:
- contacting an aqueous solution with (i) a first electrode comprising a first substrate and a first redox-species immobilized to the first substrate, wherein the first redox-species is selective toward a target functional group of the biomolecule based on a specific chemical interaction between the first redox species and the functional group, wherein the specific chemical interaction is activated by a Faradaic/redox reaction;
- and (ii) a second electrode;
- and
- applying an electrical potential across the first electrode and the second electrode such that the first redox species transforms from a first redox state to a second redox state and binds to the target functional group of the target biomolecule present in the solution,
- thereby separating the target biomolecule from the solution.
2 more elements
A method of selectively separating a first biomolecule from a second biomolecule in an aqueous solution, wherein the first biomolecule and the second biomolecule are different, the method comprising:
- contacting an aqueous solution with (i) a first electrode comprising a substrate and a first redox-species immobilized to the substrate, wherein the first redox-species is selective toward a target functional group of the first biomolecule and the second biomolecule based on a specific chemical interaction between the first redox species and the target functional group of the first biomolecule and the second biomolecule, wherein the specific chemical interaction is activated by a Faradaic/redox reaction;
- and (ii) a second electrode;
- applying an electrical potential across the first electrode and the second electrode such that the first redox species of the first electrode transforms from a first redox state to a second redox state and binds to the target functional group of the first biomolecule and the second biomolecule;
- and
- reversing the electrical potential applied in (b) across the first electrode and the second electrode such that the second biomolecule is released from the first electrode while the first biomolecule remains bound to the first electrode,
- thereby separating the first biomolecule from the second biomolecule in the aqueous solution.
3 more elements
A method of preparing an electrochemical system for biocatalysis or biosensing, the method comprising:
- contacting an aqueous solution comprising an enzyme with (i) a first electrode comprising a substrate and a first redox-species immobilized to the substrate, wherein the first redox-species is selective toward a target functional group of the enzyme based on a specific chemical interaction between the first redox species and the target functional group, wherein the specific chemical interaction is activated by a Faradaic/redox reaction;
- and (ii) a second electrode;
- and
- applying an electrical potential across the first electrode and the second electrode to activate the Faradaic/redox reaction such that the first redox species transforms from a first redox state to a second redox state and binds to the target functional group of the enzyme, wherein the enzyme remains stable during application of the electrical potential,
- thereby preparing an electrochemical system comprising the enzyme reversibly attached to the first electrode.
2 more elements
Dependencies are inferred from the claim text.