Genetic perturbation of the rna degradosome protein complex
AssigneeZymergen Inc.
InventorShawn Manchester
Life of this patent
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Abstract
The present disclosure provides novel bacterial strains with altered expression or start codon modification of one or more RNA degradation/processing genes. The RNA degradation genes of the present disclosure are controlled by heterologous promoters. The present disclosure further describes methods for generating microbial strains comprising heterologous promoter sequences operably linked to RNA degradation/processing genes.
Claims62
A genetically engineered host cell with enhanced industrial performance, said host cell comprising:
- a. a heterologous promoter polynucleotide, and
- b. a polynucleotide encoding an RNA degradation gene;
- wherein the heterologous promoter polynucleotide is operably linked to the polynucleotide encoding the RNA degradation gene.
A method for generating a host cell capable of increased biomolecule yield, the method comprising:
- a. introducing a heterologous promoter polynucleotide into the genome of the host cell, wherein the heterologous promoter polynucleotide is operably linked to a polynucleotide encoding an RNA degradation gene, thereby creating a genetically engineered host cell;
- wherein the genetically engineered host cell produces a higher biomolecule yield compared to the biomolecule yield of a control host cell cultured under identical conditions, wherein the control host cell does not comprise the heterologous promoter polynucleotide.
A method for generating a host cell capable of increased saturation biomass, the method comprising:
- a. introducing a heterologous promoter polynucleotide into the genome of the host cell, wherein the heterologous promoter polynucleotide is operably linked to a polynucleotide encoding an RNA degradation gene, thereby creating a genetically engineered host cell;
- wherein the genetically engineered host cell exhibits increased saturation biomass compared to the saturation biomass of a control host cell cultured under identical conditions, wherein the control host cell does not comprise the heterologous promoter polynucleotide.
A genetically engineered host cell with enhanced industrial performance, said host cell comprising:
- a. a polynucleotide encoding an RNA degradation gene;
- b. a mutation in the start codon of the polynucleotide of (a);
- wherein the mutation results in the replacement of the endogenous start codon of the polynucleotide with a different start codon.
A method for generating a host cell capable of increased yield of a biomolecule or increased saturation biomass, the method comprising:
- a. genetically modifying the host cell, wherein the modifying comprises mutating the start codon of an endogenous RNA degradation gene, wherein the modification generates a genetically engineered host cell;
- wherein the genetically engineered host cell has increased biomolecule yield as compared to the biomolecule yield of a control host cell, or wherein the genetically engineered host cell achieves higher saturation biomass as compared to the saturation biomass of the control host cell, wherein the control host cell does not comprise the start codon mutation of the genetically engineered host cell, and wherein the genetically engineered host cell and the control host cell are cultured under identical conditions.
Dependencies are inferred from the claim text.