Detection of dna sequences as risk factors for hiv infection

Isolated or purified DNA having a DNA sequence and a DNA length that is produced by amplifying DNA using a polymerase chain reaction, using a pair of primers selected from the group consisting of:

• (i) primers described in Appendix 2 selected from the group consisting of pair 1 (SEQ ID NOS:
• 3 and 4) and pair 2 (SEQ ID NOS:
• 5 and 6);
• and
• (ii) primers described by Appendix 3 comprising a sense primer and an antisense primer selected from the group consisting of (SEQ ID NOS:
• 7-14 or SEQ ID NOS:
• 15-23).

A method for detecting an agent that is associated with human red blood cells, comprising:

• performing a polymerase chain reaction amplification of DNA from the human red blood cells using at least one primer selected from the group consisting of SEQ ID NOS:
• 3-25 or a primer effective for use in amplifying at least twenty consecutive nucleotides of the same DNA as said primers SEQ ID NOS:
• 3-25;
• and
• detecting a selectively produced amplicon from the polymerase chain reaction amplification.

A method for detecting an agent associated with mature anucleated human red blood cells, comprising:

• extracting DNA from a sample of cells exposed to the agent associated with mature anucleated human red blood cells from a patient;
• performing a polymerase chain reaction amplification of extracted DNA, using at least one primer selected from the group consisting of SEQ ID NOS:
• 3-25 or a primer effective for use in amplifying at least twenty consecutive nucleotides of the same DNA as said primers SEQ ID NOS:
• 3-25;
• and
• detecting a selectively produced amplicon from the polymerase chain reaction amplification.