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US 2019/0194741 A1Published application
Sequencing reactions with lithium for pulse width control
AssigneePacific Biosciences of California, Inc.
InventorsAndrei Fedorov, John Lyle, Keith Bjornson, Jeremiah Hanes
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Abstract
Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having lithium that control the median pulse width for incorporated nucleotides are disclosed. The levels of lithium are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
Claims15
1-19. (canceled)
A composition for a single molecule nucleic acid sequencing reaction comprising:
- a modified recombinant phi-29 type DNA polymerase enzyme having at least 80% sequence similarity to SEQ ID NO:
- 1;
- a primer;
- a template nucleic acid;
- and
- a plurality of labeled nucleotide analogs;
- wherein the composition comprises Na at a concentration of from about 1 mM to about 400 mM.
4 more elements
Dependencies are inferred from the claim text.