Methods and compositions for mass spectrometry analysis
OwnerTianxin Wanginventor
Life of this patent
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Abstract
Methods and compounds are provided to improve the desorption and ionization of analyte for mass spectrometry analysis. More specifically, it is for electrospray ionization (ESI) mass spectrometry. The method uses charged affinity molecules that can bind with analyte either temporarily or permanently to improve the desorption and ionization of analyte. The charged affinity molecules can be positively charged or negatively charged.
Claims10
A method to improve the detection of non-ionizable analyte molecule in electrospray ionization (ESI) mass spectrometry, comprising:
- providing permanently charged affinity molecule having affinity moiety selected from cyclodextrin , calixarene, cucurbituril and pillararene that can bind with said analyte molecule via non-covalent bond;
- mixing said charged affinity molecule with a sample solution containing said analyte to form a solution containing a non-covalently bound complex between said analyte molecule and said charged affinity molecule;
- and
- performing electrospray ionization (ESI) mass spectrometry for the solution containing the bound complex , and
- detecting the presence of the analyte molecule by detecting the presence of the bound complex in ESI mass spectrometry.
A compound to improve the signal of non-ionizable analyte molecule in electrospray ionization (ESI) mass spectrometry comprising a permanently charged motif and a binding motif selected from cyclodextrin, calixarene, cucurbituril and pillararene that can bind with the said analyte molecule to form a non-covalent complex.
Dependencies are inferred from the claim text.