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AssigneeWaters Technologies Corporation
InventorMatthew A. Lauber
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Methods for preparing labeled glycosylamines from a complex matrix are provided. The methodology includes the steps of: denaturing glycoproteins in a complex matrix to form a denatured complex matrix mixture; loading the denatured complex matrix mixture onto a MWCO filtration device; adding a glycosidase enzymatic solution onto the MWCO filtration device to form a deglycosylated complex matrix mixture comprising glycosylamines; collecting glycosylamines released from the MWCO filtration device; and derivatizing glycosylamines with a rapid tagging reagent to form a plurality of labeled glycosylamines suitable for detection in various liquid chromatography systems and detectors.
A method for preparing labeled glycosylamines from a complex matrix, comprising the steps of:
A kit for preparing labeled glycosylamines comprising a MWCO filtration device, a rapid tagging reagent, a denaturing solution and an enzymatic solution, wherein the denaturing solution denatures glycoproteins in a complex matrix to form a denatured complex matrix mixture, the MWCO filtration device is configured to filter the denatured complex mixture, and the enzymatic solution is mixed and incubated with the denatured complex matrix mixture on the filtration device to yield glycosylamines, the rapid tagging reagent derivatizes the glycosylamines which are detectable in liquid chromatography, capillary electrophoresis or mass spectrometry systems.
A liquid chromatography system comprising a MWCO filtration device, a fluorescence detection device and a mass spectrometric detection device, wherein the MWCO filtration device is configured to produce glycosylamines which flow through the filtration device and are collected for derivatization by a rapid tagging reagent and subsequent fluorescence detection in the fluorescence detection device and/or mass spectrometric detection in the mass spectrometric detection device.
A liquid handling system comprising a MWCO filtration device, wherein the MWCO filtration device is configured to produce glycosylamines which flow through the filtration device and are collected for derivatization by a rapid tagging reagent and subsequent fluorescence detection in a fluorescence detection device and/or mass spectrometric detection in a mass spectrometric detection device.
Dependencies are inferred from the claim text.