Hcv core and minicore binding molecules
AssigneeIcahn School of Medicine at Mount Sinai
InventorsAndrea BRANCH, Francis ENG
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to hepatitis C virus (HCV) core and minicore-binding molecules and nucleic acid sequences encoding such molecules. In particular embodiments, the present invention provides HCV core and minicore-binding molecules (e.g., monoclonal antibodies or antibody fragments) with particular light chain and/or heavy chain CDRs (e.g., selected from SEQ ID NOS: 2-4 and 6-8) and methods for using such molecules to detect the presence of HCV core proteins (e.g., mature p21 core protein or minicore proteins) in a sample.
Claims25
A hepatitis C virus (HCV) core protein-binding molecule, comprising:
- a) a light chain variable region comprising
- i) a first light chain complementarity determining region (CDRL1) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, and SEQ ID NO:
- 2 with one or more conservative amino acid changes;
- ii) a second light chain CDR (CDRL2) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:3 and SEQ ID NO:3 with one or more conservative amino acid changes;
- and
- iii) a third light chain CDR (CDRL3) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:4 and SEQ ID NO:4 with one or more conservative amino acid changes;
- and
- b) a heavy chain variable region, comprising
- i) a first heavy chain CDR (CDRH1) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:6 and SEQ ID NO:6 with one or more conservative amino acid changes;
- ii) a second heavy chain CDR (CDRH2) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:7 with one or more conservative amino acid changes;
- and
- iii) a third heavy chain CDR (CDRH3) consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:8 and SEQ ID NO:8 with one or more conservative amino acid changes.
A nucleic acid comprising:
- a) a first nucleic acid sequence encoding a light chain variable region, wherein said light chain variable region comprises
- i) a CDRL1 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2 and SEQ ID NO:
- 2 with one or more conservative amino acid changes;
- ii) a CDRL2 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO:
- 3 with one or more conservative amino acid changes;
- and
- iii) a CDRL3 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4 and SEQ ID NO:
- 4 with one or more conservative amino acid changes;
- and
- b) a second nucleic acid sequence encoding a heavy chain variable region, wherein said heavy chain variable region comprises;
- i) a CDRH1 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:6 and SEQ ID NO:6 with one or more conservative amino acid changes;
- ii) a CDRH2 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:7 with one or more conservative amino acid changes;
- and
- iii) a CDRH3 consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:8 and SEQ ID NO:8 with one or more conservative amino acid changes.
24-28. (canceled)
Dependencies are inferred from the claim text.