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OwnerDavid Anderson, Riya Palchaudhuri +2inventors
InventorsDavid Anderson, Riya Palchaudhuri, Suzanne Crowe, Clovis Palmer
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An immunoassay for assisting in the diagnosis of sepsis or severe infection in a patient/subject, the assay comprising the steps of: (i) optionally contacting a test sample comprising neutrophils from the patient with an agent that permeabilises or solubilises neutrophils; (ii) simultaneously with (i) or sequentially, contacting the sample with a binding agent that binds specifically to CD64 in the sample and forms a CD64-binding agent complex a; (iii) simultaneously with (i) and/or (ii) or sequentially, contacting the sample with a second binding agent that binds specifically to a neutrophil number marker (NNM) in the sample and forms a neutrophil marker-binding agent complex b; (iv) employ the amount of complex a and complex b to determine the relative level of CD46 and of NNM in the sample.
An immunoassay for evaluating test sample neutrophil activation suitable for assisting in the diagnosis of sepsis or severe infection, the assay comprising the steps of:
An immunoassay for assisting in the diagnosis of sepsis or severe infection in a patient/subject, the assay comprising the steps of:
An immunoassay for assisting in the diagnosis or prognosis of sepsis or severe infection in a patient/subject, the assay comprising the steps of:
A kit or component of a diagnostic comprising (i) an immunoassay (such as a POC) device comprising a microchannel or porous membrane operably connected to a sample portion, two or more capture (test) portions, and optionally one or more of the following;
An assay step for enhancing the sensitivity and/or specificity of an assay that evaluates sample neutrophil (leukocyte) activation by measuring the level of cell surface CD64 on the surface of neutrophils (leukocytes) in a sample, the step comprising measuring the total level of CD64 in the sample by permeabilising/solubilizing neutrophils (leukocytes) to allow for detection of intracellular as well as surface CD64 (total CD64) in the sample using a CD64 binding agent.
An assay step for enhancing the sensitivity and/or specificity of an assay that evaluates sample neutrophil (leukocyte) activation by measuring the level of cell surface CD64 on the surface of neutrophils (leukocyte) in a sample, the step comprising measuring the total level of CD64 and NNM in the sample by permeabilising/solubilizing neutrophils (leukocyte) to allow for detection of intracellular as well as surface CD64 and NNM in the sample.
Dependencies are inferred from the claim text.