Micropatterning assembly, methods for micropatterning, and micropatterned devices
AssigneeIst Austria (Institute of Science and Technology Austria)
InventorsMICHAEL SIXT, Robert HAUSCHILD, Alexander Jan SCHWARZ, Maria NEMETHOVA
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Abstract
The present invention relates to the field of micropatterning. In particular, the present invention provides micropatterning assemblies and methods for micropatterning. Moreover, the present invention provides micropatterned devices obtained by using the micropatterning assemblies and/or methods of the invention. Furthermore, the present invention provides methods for using said devices.
Claims55
An assembly for micropatterning a transparent solid carrier, the assembly comprising:
- i) a stage adapted for mounting a transparent solid carrier thereto;
- ii) a spatially light modulating optics comprising
- a) a light source for providing light to said carrier along an optical path, wherein the light source comprises one or more LEDs;
- b) a spatial light modulator (SLM) for generating a binary or greyscale pattern image of light, wherein the SLM is positioned in said optical path between said light source and said carrier;
- c) first optical means for directing light from said light source along the optical path to said SLM;
- and
- d) second optical means comprising an objective for directing the pattern image generated by said SLM along the optical path to said carrier such that the pattern image is projected onto said carrier;
- and
- iii) control means having a digital input and connected to said SLM for providing control signals to said SLM to cause said SLM to generate a pattern image of light.
The assembly of any of the preceding claims, wherein the SLM is selected from DMD and LCD.
The assembly of any of the preceding claims, wherein the one or more LEDs have a wavelength of 302 nm, 365 nm, 470 nm, or 560 nm.
The assembly of any of the preceding claims, comprising at least three LEDs with distinct wavelengths.
The assembly of any of the preceding claims, wherein at least one LED has a wavelength in the UV range, wherein preferably at least one LED has a wavelength of 302 nm or 365 nm.
The assembly of any of the preceding claims, wherein the optical path does not comprise any observation optics.
The assembly of any of the preceding claims, wherein the assembly does not comprise any optical filters.
The assembly of any of the preceding claims, wherein all components are arranged integrally within the assembly.
A method for micro-patterning a solid carrier, the method comprising:
- i) providing a transparent solid carrier, wherein one surface side of said carrier is covered with a liquid phase comprising adaptor molecules;
- ii) projecting a desired light pattern onto the carrier/liquid interface using an assembly comprising the following elements:
- a) a stage adapted for mounting a specimen thereto;
- b) a spatially light modulating optics comprising:
- a light source for providing light to the carrier along an optical path;
- a spatial light modulator (SLM) for generating a binary or greyscale pattern image of light, wherein the SLM is positioned in said optical path between said light source and said carrier;
- first optical means for directing light from said light source along the optical path to said SLM;
- and
- second optical means comprising an objective for directing the pattern image generated by said SLM along the optical path to said carrier such that the pattern image is projected onto said carrier;
- c) control means having a digital input and connected to said SLM for providing control signals to said SLM to cause said SLM to generate a pattern image of light, whereby the adaptor molecules are covalently attached to the solid carrier surface by photo-immobilization according to the projected light pattern;
- and
- iii) attaching a coupling molecule to the adaptor molecule.
A cell binding device offering defined patterns comprising
- a) a passivating polymeric coating that is covalently attached to the surface of a solid carrier;
- b) an adaptor molecule covalently bound by directed photo-immobilization to a predetermined area of the surface coating, and
- c) a cell binding molecule covalently bound to said adaptor molecule.
Dependencies are inferred from the claim text.