Immuno chromatography method with centrifuge isolation

A combined kit of immune chromatography fluorescence assay with centrifuge isolation, comprising:

• an analyte-specific binding substance,
• a micro particle,
• a solid phase membrane,
• a fluorescent substance,
• a centrifugation device,
• and a fluorescence detector;
• wherein the fluorescent substance comprises at least one of an organic fluorescent dye and a rare-earth element fluorescent dye;
• the micro particle is able to form a stable non-specific binding to a protein and/or the fluorescent substance directly and/or by chemical crosslinking;
• the solid phase membrane is a membranous substance with a non-specific binding characteristic to a protein;
• the centrifugation device centrifugally drives a liquid phase to flow on the solid phase membrane through chromatography;
• and
• the analyte-specific binding substances selected from at least one of substances having a specific binding ability comprising an antigen, an antibody, an avidin and a biotin.

A combined kit of immune chromatography colloidal gold assay with centrifuge isolation, comprising an analyte-specific binding substance, a colloidal gold micro particle, a solid phase membrane, a centrifugal device and a colloidal gold detector;

• wherein the colloidal gold micro particle is prepared using a gold solution and forms a stable non-specific binding to a protein directly;
• the solid phase membrane is membranous substance with a non-specific binding characteristic to a protein;
• the centrifugal device centrifugally drives a liquid phase to flow on the solid phase membrane through chromatography;
• the analyte-specific binding substance is selected from at least one of substances having a specific binding ability comprising an antigen, an antibody, an avidin and a biotin;
• and
• the colloidal gold detector is used to perform a quantitative or semi-quantitative detection through colorimetric analysis of a colloidal gold color.

A centrifugal separation and detection device, comprising:

• a sampling member,
• a solid phase membrane,
• a centrifugal device,
• and a detector;
• wherein the centrifugal device comprises a centrifugal rotor driven by a drive motor and a support base, and the centrifugal rotor is supported by the support base;
• the sampling member is not directly connected to the centrifugal rotor, and is arranged above, below or outside the centrifugal rotor;
• the sampling member comprises a liquid phase storage device, an injection tube and an injection pump;
• and the liquid phase storage device is communicated with the injection tube;
• and a liquid in the liquid phase storage device is driven to enter into the injection tube with the injection pump;
• the solid phase membrane is arranged on the centrifugal rotor and a liquid phase sample is loaded directly or indirectly to a proximal side of the solid phase membrane with the injection tube;
• and
• the detector is not connected directly to the centrifugal rotor and is arranged above, below or outside the centrifugal rotor.