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US 2019/0170695 A1Published application
System and Method for Nanoscale Photoacoustic Tomography
AssigneeThe Board of Regents of the University of Oklahoma
InventorsLiangzhong Xiang, Jian Chen, Pratik Samant
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Abstract
A method and system of nanoscale photoacoustic tomography (nPAT) for non-invasive three-dimensional mapping and characterization of fine cellular structures (such as but not limited to organelles, vesicles, and macromolecules) of biological samples is disclosed.
Claims30
A nanoscale photoacoustic tomography system comprising:
- an ultrafast pulse laser system configured to:
- provide a generation beam for generating photoacoustic waves in a biological sample;
- and
- provide a probe beam for assessing changes in light reflectivity in the biological sample caused by the photoacoustic waves;
- a scanning stage configured to mount the biological sample for analysis;
- a first focusing assembly configured to focus the generation beam on the biological sample;
- a second focusing assembly configured to:
- focus the probe beam on the biological sample;
- and
- collect backscattered light reflected from the biological sample;
- an optical detector configured to:
- sense the backscattered light;
- and
- emit signals in response to the backscattered light;
- and
- a processor configured to record and process the signals.
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A method of nanoscale photoacoustic tomography comprising:
- emitting, using an ultrafast pulse laser system, a generation beam for generating photoacoustic waves in a biological sample and a probe beam for assessing changes in light reflectivity in the biological sample;
- illuminating the biological sample with the generation beam to form the photoacoustic waves in the biological sample, wherein the photoacoustic waves cause changes in the light reflectivity of the biological sample;
- illuminating the biological sample with the probe beam at a predetermined delayed time after illuminating the biological sample with the generation beam;
- detecting, via an optical detector, backscattered light reflected from the biological sample after illumination with the probe beam;
- and
- recording and processing signals from the optical detector to form an image of at least a portion of the biological sample.
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A nanoscale photoacoustic tomography system comprising:
- an ultrafast pulse laser system configured to:
- provide a generation beam for generating photoacoustic waves in a biological sample;
- and
- provide a probe beam for assessing changes in light reflectivity in the biological sample caused by the photoacoustic waves;
- a scanning stage configured to mount the biological sample for analysis;
- an objective lens configured to:
- focus the generation beam on the biological sample;
- focus the probe beam on the biological sample;
- and
- collect backscattered light reflected from the biological sample;
- and
- an optical detector configured to:
- sense the backscattered light;
- and
- emit signals in response to the backscattered light.
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Dependencies are inferred from the claim text.