Methods of particle manipulation and analysis
AssigneeThe Trustees of Princeton University
InventorsJesse AULT, Sangwoo Shin, Howard A. Stone, Jie Feng, Patrick Warren
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Abstract
Methods described herein, in some embodiments, permit extraction of particle structural and/or surface charge data from gradient induced particle motion in channels. In one aspect, a method of manipulating particle motion comprises introducing a fluid into a channel, the fluid comprising particles, and driving particle accumulation to a preselected location in the channel by setting advective velocity of the fluid to offset diffusiophoretic mobility of the particles at the preselected location.
Claims37
A method of manipulating particle motion comprising:
- introducing a fluid into a channel, the fluid comprising particles;
- and
- driving particle accumulation to a preselected location in the channel by setting advective velocity of the fluid to offset diffusiophoretic velocity of the particles at the preselected location.
A method of particle separation comprising:
- introducing a fluid into a channel, the fluid comprising a mixture of particle species;
- and
- separating the particle species in the fluid by driving accumulation of the particle species to preselected locations in the channel via setting advective velocity of the fluid to offset diffusiophoretic velocity of a particle species at each of the preselected locations.
A method of particle analysis comprising:
- introducing a fluid sample into a channel;
- determining the presence of analyte particles in the fluid sample by driving accumulation of the analyte particles to a preselected location in the channel via setting advective velocity of the fluid sample to offset diffusiophoretic velocity of the analyte particles at the preselected location;
- and
- detecting the presence of the analyte particles at the preselected location.
A method of particle analysis comprising:
- introducing a fluid sample into a channel;
- determining the presence of a plurality of analyte particle species in the fluid sample by driving accumulation of the analyte particle species to preselected locations in the channel via setting advective velocity of the fluid to offset diffusiophoretic velocity of an analyte particle species at each of the preselected locations;
- and
- detecting the presence of the analyte particle species at each of the preselected locations.
A method of determining particle zeta potential comprising:
- introducing a fluid into a channel, the fluid having an advective velocity and solute concentration gradient;
- introducing particles into the fluid, and accumulating the particles at a location in the channel where the advective velocity of the fluid is offset by diffusiophoretic velocity of the particles;
- calculating the diffusiophoretic mobility of the particles from the location in the channel;
- and
- deriving zeta potential of the particles from the calculated diffusiophoretic mobility.
A method of determining particle zeta potential comprising:
- providing at least one dead-end pore containing a solute having a first solute concentration;
- introducing colloidal particles having positive diffusiophoretic mobility and a second solute concentration that is less than the first solute concentration;
- measuring the image intensity in the dead-end pore;
- determining a maximum colloidal density based on the image intensity;
- and
- determining the particle zeta potential based on the maximum colloidal density.
A method of determining zeta potential of channel walls comprising:
- establishing a solute concentration gradient to induce a wall slip boundary condition in the channel;
- measuring pressure drop over the channel length;
- determining channel wall diffusiophoretic mobility of the slip boundary condition from the measured pressure drop;
- and
- deriving the zeta potential of the channel walls from the wall diffusiophoretic mobility.
A zeta potentiometer comprising:
- at least one flow channel;
- at least one light source for illuminating the flow channel;
- an imaging device positioned to observe the illuminated flow channel;
- and
- a sample storage location adapted to provide sample to the at least one flow channel.
Dependencies are inferred from the claim text.