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US 2019/0169700 A1Published application
Methods and Systems for Detecting Biological Components
AssigneeThe Regents of the University of California
InventorsAdam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Abstract
Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.
Claims12
-146. (canceled)
A method of synthesizing a target polynucleotide, the method comprising:
- contacting a polynucleotide-containing component from a sample with lysis reagents in a droplet, the lysis reagents comprising an enzyme having protease activity, wherein the droplet is encapsulated with an immiscible carrier fluid;
- moving the droplet into a collection reservoir;
- incubating the droplet in the collection reservoir for a first duration and then inactivating the enzyme having protease activity;
- adding to the droplet a nucleic acid synthesis reagent to form a nucleic acid synthesis droplet in the immiscible carrier fluid to form a nucleic acid droplet;
- and
- synthesizing the target polynucleotide within the nucleic acid synthesis droplet.
3 more elements
Dependencies are inferred from the claim text.