Hybrid nanopores and uses thereof for detection of analytes

A method for analysis of at least one analyte in a sample comprising:

• (i) applying a sample comprising at least one analyte or suspected to comprise at least one analyte onto a hybrid structure, wherein the hybrid structure comprises (a) a solid substrate having at least one nanopore perforating therethrough, and (b) at least one ring-like polypeptide situated at a region of said at least one nanopore, said region being selected from an opening of the nanopore and an interior region of said nanopore, wherein the binding of the at least one ring-like polypeptide to the nanopore is different from covalent binding,
• (ii) permitting the sample to flow through the nanopore;
• (iii) determining at least one of (a) presence or absence of an analyte in the sample, (b) identity of the analyte in the sample and (c) concentration of the analyte in the sample.