METHOD FOR MEASURING HbA1c

A method of measuring a ratio of a hemoglobin A1c concentration to a hemoglobin concentration in a sample by an enzymatic method, the method comprising:

• a first step of optically measuring the hemoglobin concentration;
• and
• a second step of optically measuring the hemoglobin A1c concentration,
• wherein, when HbA1c % is calculated by dividing the hemoglobin A1c concentration measured in the second step by the hemoglobin concentration measured in the first step, the hemoglobin concentration serving as a denominator, which is measured at a first wavelength, is corrected using a result measured at a second wavelength that satisfies the following conditions:
• a longer wavelength than 480 nm;
• a wavelength that gives an absorbance equal to or less than 1/10 of an absorbance at 480 nm for a 100 μmol/L solution of hemoglobin in physiological saline;
• and
• a wavelength at which a correlation exists between a fat particle concentration and an absorbance.