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US 2019/0169640 A1Published application
Novel dna-binding proteins and uses thereof
AssigneeSangamo Therapeutics, Inc.
InventorsPhilip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
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Abstract
Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Claims12
An isolated polynucleotide encoding a non-naturally occurring TALE DNA-binding polypeptide comprising:
- two or more TALE-repeat units, the TALE repeat units comprising a repeat variable di-residue (RVD);
- and wherein the N-terminal region lacks at least 152 amino acids of a full-length TALE protein and the C-terminal region is truncated to residue C+63, and further wherein the polypeptide binds to DNA.
Dependencies are inferred from the claim text.