Genetic erasers
AssigneeSenti Biosciences, Inc.
InventorsTimothy Kuan-Ta Lu, Remus Wong
Life of this patent
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Abstract
Provided herein, in some embodiments, are methods, compositions, systems and kits that enable removal of heterologous nucleic acid from engineered cells.
Claims120
An engineered genetic construct comprising a cassette that comprises (a) a nucleotide sequence encoding a first product of interest, and (b) a nucleotide sequence encoding a second product of interest and a counterselectable marker, wherein expression or activity of the first product of interest is activatable, and wherein the first product of interest modulates excision or degradation of the cassette.
An engineered genetic construct comprising a cassette that comprises:
- (a) an inducible promoter operably linked to a nucleotide sequence encoding a recombinase;
- and
- (b) a promoter operably linked to a nucleotide sequence encoding a product of interest and a counterselectable marker,
- wherein the cassette is flanked by cognate recombinase recognition sites.
An engineered genetic construct comprising a cassette that comprises:
- (a) an inducible promoter operably linked to a nucleotide sequence encoding a nuclease;
- and
- (b) a promoter operably linked to a nucleotide sequence encoding a product of interest and a counterselectable marker,
- wherein the cassette comprises cognate nuclease recognition sites, optionally flanking the cassette.
An engineered genetic construct comprising a cassette that comprises:
- (a) at least one promoter operably linked to at least one nucleotide sequence encoding at least one ligand-dependent chimeric recombinase;
- and
- (b) at least one promoter operably linked to a nucleotide sequence encoding a product of interest and a counterselectable molecule,
- wherein the cassette is flanked by cognate recombinase recognition sites.
An engineered genetic construct comprising a cassette that comprises:
- (a) a promoter operably linked to a nucleotide sequence encoding a first fragment of a recombinase;
- (b) a promoter operably linked to a nucleotide sequence encoding a second fragment of a recombinase, wherein the first fragment and the second fragment when combined form a full-length functional recombinase;
- and
- (c) a promoter operably linked to a nucleotide sequence encoding a product of interest and a counterselectable marker,
- wherein the cassette is flanked by cognate recombinase recognition sites.
Dependencies are inferred from the claim text.