Algal lipid productivity via genetic modification of a signaling protein
AssigneeSynthetic Genomics, Inc.
InventorImad Ajjawi
Life of this patent
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Abstract
The present invention provides mutant microorganisms having attenuated expression of a gene encoding a polypeptide that includes a GAF domain wherein the mutant microorganisms have higher lipid productivity and/or exhibit increased partitioning of carbon to lipid as compared to wild-type microorganisms from which they are derived. Also provided are methods of producing lipids using the mutant microorganisms, guide RNAs, and nucleic acid constructs used for producing mutant microorganisms.
Claims58
A mutant microorganism having attenuated expression of a gene encoding a polypeptide that comprises a GAF domain, wherein the mutant microorganism:
- a) produces at least about 25% more lipid than a control microorganism;
- and/or
- b) exhibits increased partitioning of carbon to lipid with respect to the control microorganism;
- when the mutant microorganism and control microorganism are cultured under identical conditions.
A guide RNA of a CRISPR system, wherein the guide RNA comprises a sequence corresponding to SEQ ID NO:4.
A nucleic acid construct for homologous recombination comprising a nucleotide sequence from or adjacent to a naturally-occurring algal gene encoding a polypeptide having an amino acid sequence with at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:1 or SEQ ID NO:2;
- a gene localized to the Naga_100020g79 locus;
- and/or a gene that comprises an ORF comprising a sequence having at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:3 and/or SEQ ID NO:4.
A nucleic acid construct for expression of an antisense RNA, shRNA, microRNA, or ribozyme comprising a nucleotide sequence complementary to at least a portion of a naturally-occurring gene encoding a polypeptide having an amino acid sequence with at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:1 or SEQ ID NO:2;
- a gene localized to the Naga_100020g79 locus;
- and/or a gene that comprises an ORF comprising a sequence having at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:3 and/or SEQ ID NO:4.
A nucleic acid molecule encoding a guide RNA of a CRISPR system, wherein the guide RNA comprises at least a portion of a naturally-occurring algal gene encoding a polypeptide having an amino acid sequence with at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:1 or SEQ ID NO:2;
- a gene localized to the Naga_100020g79 locus;
- and/or a gene that comprises an ORF comprising a sequence having at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% identity to SEQ ID NO:3.
Dependencies are inferred from the claim text.