Low inoculum, long co-culture agrobacterium-mediated transformation of plants
AssigneeOhio State Innovation Foundation
InventorsJohn James FINER, Zhifen ZHANG
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AssigneeOhio State Innovation Foundation
InventorsJohn James FINER, Zhifen ZHANG
Estimating the term…
Disclosed herein is a method for producing genetically modified plants comprising inoculating a plant, plant cell, plant seed, or plant tissue with an Agrobacterium containing a nucleic acid of interest at an inoculum density of about 10-10,000 CFU/ml; and culturing the Agrobacterium-inoculated plant, plant cell, plant seed, or plant tissue for a period of from 7 days to 28 days, wherein said culturing results in a genetically modified plant, plant cell, plant seed, or plant tissue. Also disclosed are genetically modified plants, plant cells, plant seeds, or plant tissue based on the methods disclosed herein, as well as their progeny, and propagation material related thereto.
A method for producing a genetically modified plant comprising
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