Recognition tags for tgase-mediated conjugation
AssigneesInnate Pharma, Paul Scherrer Institut
InventorsEliane Fischer, Francois Romagne, Patrick Dennler
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Abstract
The present application relates to methods for the functionalization of antibodies using transglutaminase, in particular antibodies lacking Fc regions. Also disclosed herein are peptide tags for transglutaminase, linking reagents, functionalized antibodies, multi-specific antibodies, pharmaceutical compositions, and method of treating disease and/or conditions.
Claims10
1-46. (canceled)
A polypeptide comprising a TGase recognition tag comprising a c-myc tag peptide sequence, wherein said TGase recognition tag comprises an acceptor glutamine residue functionalized with a moiety-of-interest via a lysine based linker.
A method for making a polypeptide conjugate, the method comprising reacting a polypeptide comprising a TGase recognition tag comprising a c-myc tag peptide sequence, with a lysine-based linker or polypeptide comprising a lysine residue, in the presence of a TGase and under conditions sufficient such that a polypeptide conjugated to a lysine-based linker or polypeptide comprising a lysine residue is obtained.
Dependencies are inferred from the claim text.