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US 2019/0169578 A1Published application
Methods and compositions for production of vaccina virus
AssigneeSillajen Biotherapeutics, Inc.
InventorsDavid KIRN, John BELL
Life of this patent
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Abstract
Certain embodiments are directed to viral production processes for the large scale production of vaccinia virus.
Claims19
A method for producing a vaccinia virus comprising:
- (a) infecting HeLa cells adhered to a cell culture bioreactor surface with a Western Reserve strain vaccinia virus by contacting the HeLa cells with the vaccinia virus at a multiplicity of infection (m.o.i.) of between 0.005 and 1.0 plaque forming units (pfu)/cell;
- (b) culturing the infected cells in an infection medium having a pH of 7.2 to 7.6 at temperature of 30° C. to 40° C.;
- and
- (c) harvesting vaccinia virus from the culture,
- whereby at least 50 pfu of vaccinia virus per HeLa cell is produced, calculated as
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- HeLa
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54 more elements
The method of claim 18, wherein the vaccinia virus is a recombinant vaccinia virus, lacking a functional thymidine kinase gene and/or lacking a functional vaccinia growth factor gene and/or encoding a granulocyte macrophage colony-stimulating factor polypeptide.
Dependencies are inferred from the claim text.