Method for producing cultivated epithelial cell sheet
AssigneeKyoto Prefectural Public University Corporation
InventorsTakahiro NAKAMURA, Shigeru KINOSHITA, Seiichi YOKOO, Satoru YAMAGAMI
Life of this patent
Estimating the term…
Abstract
A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.
Claims6
1-4. (canceled)
A method for producing an epithelial cell sheet in which cells that express an epithelial stem cell marker, a proliferating cell marker, and corneal epithelial cell marker keratin 12 and maintain a property as stem cells are present, the method comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium,
- wherein the serum-free medium comprises
- (i) EGF protein or KGF protein,
- (ii) B-27 supplement,
- (iii) a ROCK inhibitor, and
- (v) one or more catechin.
A serum-free medium for producing an epithelial cell sheet in which cells that express an epithelial stem cell marker, a proliferating cell marker, and corneal epithelial cell marker keratin 12 and maintain a property as stem cells are present, using cells derived from oral mucosal epithelial cells, the medium comprising
- (i) EGF protein or KGF protein,
- (ii) B-27 supplement, and
- (iii) a ROCK inhibitor.
Dependencies are inferred from the claim text.