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US 2019/0169564 A1Published application
Methods of culturing microorganisms in non-axenic mixotrophic conditions
AssigneeHeliae Development, LLC
InventorsENEKO GANUZA TABERNA, ANNA LEE TONKOVICH, JASON D. LICAMELE
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Abstract
Methods of culturing microorganisms in non-axenic mixotrophic conditions are disclosed. A method of culturing microalgae mixotrophically and controlling bacterial contamination with an acetic acid/pH auxostat system is specifically described. Methods of culturing microalgae mixotrophically with an increased productivity through an increase in oxygen transfer to the culture are also described.
Claims9
A method of culturing Chlorella in non-axenic mixotrophic culture conditions, comprising:
- (a) inoculating an aqueous culture medium with a culture comprising Chlorella in an open culturing vessel;
- (b) supplying light comprising photosynthetically active radiation (PAR) in a photoperiod in the range of 10-16 hours of light for each 24 hour day;
- (c) activating a pH auxostat system with an initial concentration of sodium acetate in the range of 0.05-10 g/L in the aqueous culture medium to start supplying acetic acid, at a concentration in the range of 15-50% (v/v) to the culture comprising Chlorella, wherein the pH auxostat system operates with in the range of 6-9, and the temperature ranges from 10° C. to 30° C.;
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- (d) maintaining a dissolved oxygen (DO) concentration in the range of 1-6 mg O2/Liter.
2 more elements
Dependencies are inferred from the claim text.