Cell retention device and method
AssigneeBoehringer Ingelheim International GmbH
InventorsJonathan COFFMAN, Scott GODFREY, Samantha WANG
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Abstract
The subject technology relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 μm and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by more than 20%.
Claims21
A cell retention device comprising a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 μm.
A method for harvesting recombinant protein products from a perfusion culture vessel, said method comprising the steps of:
- a. subjecting cell culture fluid of a perfusion culture vessel to a cell retention device to be filtered, wherein the cell culture fluid comprises cells and recombinant protein products produced by said cells and wherein the cell retention device comprises a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 μm;
- b. collecting filtrate from the cell retention device, wherein the filtrate comprises the recombinant protein products;
- c. Recirculating the filtered cell culture fluid to the perfusion culture vessel.
21-46. (canceled)
Dependencies are inferred from the claim text.