Three-dimensional structuring method and three-dimensional structuring system of cells

A three-dimensional culture system of cells, comprising:

• a flow path spatial part in which a space surrounded by an inner wall surface from a supply port to a discharge port of a culture solution is used as a flow path;
• a plurality of fibers which are positioned so that a longitudinal direction of each of the fibers is along a direction of the flow path while a predetermined interval is mutually maintained between the fibers;
• and
• a liquid supplying part which supplies the culture solution in the flow path from the supply port of the flow path spatial part,
• wherein a plurality of cell clusters are placed in the flow path upon causing the cell clusters to run with a liquid flow of the culture solution supplied in the flow path from the supply port, and each of the cell clusters is cultured by stacking the cell clusters on an outer surface of each of the fibers with a vicinity of the discharge port as a growth origin.