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US 2019/0169546 A1Published application
Detergent composition comprising protease and amylase variants
AssigneeNovozymes A/S
InventorsCarsten Andersen, Yanfei Wang, Wenwen Tao, Bitten Plesner, Elena Genesca Pont
Life of this patent
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Abstract
The present invention relates to detergent compositions comprising protease variants and alpha-amylases or variants thereof. Furthermore, the present invention relates to methods of using the detergent compositions.
Claims17
A detergent composition comprising
- (i) at least one alpha-amylase variant comprising a modification in one or more positions corresponding to positions 1, 54, 56, 72, 109, 113, 116, 134, 140, 159, 167, 169, 172, 173, 174, 181, 182, 183, 184, 189, 194, 195, 206, 255, 260, 262, 265, 284, 289, 304, 305, 347, 391, 395, 439, 469, 444, 473, 476, or 477 of SEQ ID NO:
- 1, wherein said alpha-amylase variant has a sequence identity of at least 75% but less than 100% to SEQ ID NO:
- 1 and wherein said alpha-amylase variant has alpha-amylase activity;
- and
- (ii) at least one protease having protease activity, wherein said protease is selected from the group of:
- (a) a protease having a sequence identity of at least 70% to the sequences of SEQ ID NOs:
- 2, 3, 19, 20, or 23;
- (b) a protease variant comprising a substitution at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO:
- 2, wherein said protease variant has a sequence identity of at least 75% but less than 100% to SEQ ID NO:
- 2;
- (c) a protease variant comprising a modification in one or more positions corresponding to positions 32, 33, 48, 49, 50, 51, 52, 53, 54, 58, 59, 60, 61, 62, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 116, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 150, 152, 153, 154, 155, 156, 158, 159, 160, 161, 164, 169, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 197, 198, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, or 216 as compared to the protease in SEQ ID NO:3, wherein said protease variant has at least 75% sequence identity to SEQ ID NO:
- 3,
- (d) a protease variant comprising a substitutions in one or more positions corresponding to positions 9, 15, 27, 42, 52, 55, 56, 59, 60, 66, 74, 85, 97, 99, 101, 102, 104, 116, 118, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 198, 199, 200, 203, 206, 210, 211, 212, 216, 230, 232, 239, 242, 250, 253, 255, 256, or 269, wherein numbering is according to SEQ ID NO:
- 3, wherein said protease variant has at least 60% sequence identity to SEQ ID NO:
- 3, and
- (e) a protease variant comprising a substitution in one or more positions corresponding to positions 32, 33, 49, 50, 51, 52, 53, 54, 55, 60, 61, 62, 63, 64, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 118, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 152, 154, 155, 156, 157, 158, 161, 162, 163, 167, 170, 175, 181, 187, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 203, 204, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, or 222 as compared to the protease shown in SEQ ID NO:
- 23, wherein said protease variant has at least 75% sequence identity to SEQ ID NO:
- 23.
15 more elements
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Dependencies are inferred from the claim text.