T cell receptors and uses thereof
AssigneeMedigene Immunotherapies GmbH
InventorsChristian ELLINGER, Carina WEHNER, Manon WEIS, Susanne WILDE, Dolores SCHENDEL
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Abstract
The present invention relates to the field of biotechnology. Specifically, the invention provides antigen-specific T-cell receptors (TCRs). Further, the invention encompasses polynucleotides encoding the same and vectors comprising said polynucleotides. Host cells comprising the molecules of the invention are also provided. Moreover, the invention provides means and methods for diagnostics and therapy, in particular of cancer.
Claims24
A T-cell receptor (TCR) comprising:
- (i) a CDR3 of the TCR alpha chain variable region comprising or consisting of the amino acid sequence of CAVHSTAQAGTALIF (SEQ ID NO:
- 1)
- or an amino acid sequence having at least 80% identity to SEQ ID NO:
- 1, more preferably at least 85% identity, more preferably 90% or 95%
- and/or
- (ii) a CDR3 of the TCR beta chain variable region comprising or consisting of the amino acid sequence of CASSTHRGQTNYGYTF (SEQ ID NO. 2)
- or an amino acid sequence having at least 80% identity to SEQ ID NO:
- 2, more preferably at least 85% identity, more preferably 90% or 95% identity,
- said TCR being capable of binding to the epitope comprised within the amino acid sequence of X1LX2GLDX3LL (SEQ ID NO:31) or its MHC-bound form, preferably to the epitope comprised within the amino acid sequence of VLDGLDVLL (SEQ ID NO:32) or its MHC-bound form.
The TCR according to any one of the preceding claims, comprising
- (i) a TCR alpha chain comprising or consisting of an amino acid sequence selected from SEQ ID NO:
- 7;
- SEQ ID NO:
- 9, SEQ ID NO:
- 11, or SEQ ID NO:
- 13,
- or an amino acid sequence having at least 80% identity, more preferably at least 85% identity, more preferably 90% or 95% to SEQ ID NO:
- 7, 11, 9 or 13;
- and/or
- (ii) a TCR beta-chain comprising or consisting of an amino acid sequence selected from of SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, or SEQ ID NO:
- 14
- or an amino acid sequence having at least 80% identity, more preferably at least 85% identity, more preferably 90% or 95% to SEQ ID NO:
- 8, 10, 12 or 14.
The TCR according to any one of the preceding claims, said TCR being selected from a native TCR, a TCR variant, a TCR fragment, or a TCR construct.
The TCR according to any of the preceding claims, further comprising one or more fusion component(s) optionally selected from Fc receptors;
- Fc domains, including IgA, IgD, IgG, IgE, and IgM;
- cytokines, including IL-2 or IL-15;
- toxins;
- antibodies or antigen-binding fragments thereof, including anti-CD3, anti-CD28, anti-CDS, anti-CD 16 or anti-CD56 antibodies or antigen-binding fragments thereof;
- CD247 (CD3-zeta), CD28, CD137, CD134 domain, or combinations thereof, optionally further comprising at least one linker.
The TCR according to any one of the preceding claims, further comprising at least one label.
The TCR according to any one of the preceding claims which is soluble.
A nucleic acid encoding the TCR according to any one of the preceding claims.
Dependencies are inferred from the claim text.