Acylated derivative of human insulin or analogue thereof

An acylated derivative of human insulin analogue or a zinc complex thereof, wherein the acylated derivative of human insulin analogue has a structure represented by the following formula:

• S—W—X—Y—Z (I)
• wherein S is human insulin in which threonine at amino acid 30 on B chain is absent;
• and
• —W—X—Y—Z is an acylated modifying group of insulin analogue;
• wherein W is a diacyl structure having —OC(CH2)nCO—, wherein n is an integer between 2 and 10, and an amide bond is formed by one of said diacyl structure's acyl groups with α-amino group of the amino acid residue at N-end of A-chain or B-chain of parent insulin or analogue thereof or with ε-amino group of lysine residue present on B-chain;
• X is a diamino compound containing carboxylic acid group, and an amide bond is formed by linking one of the amino groups of the compound to one of the acyl groups in W;
• Y is -A(CH2)m—, wherein m is an integer between 6 and 32, preferably an integer between 10 and 16, particularly preferably an integer between 12 and 14, and A is absent or CO—;
• and
• Z is —COOH.