US 2019/0169238 A1 · Improved Filamentous Fungal Host

Abstract

The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.

Claims17

Dependent claims

A filamentous fungal host cell producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16.

A method of improving the productivity or yield of a secreted polypeptide of interest in a filamentous fungal host cell, said method comprising the steps of:

a) providing a filamentous fungal host cell comprising and expressing an ireA gene or a homologue thereof encoding a IreA polypeptide or a homologue thereof;
and
b) mutating the ireA gene or homologue thereof to provide a mutated ireA gene or homologue thereof that encodes a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16.

Dependencies are inferred from the claim text.

Facts

Application

16/319,780Filed 19 Jul 2017

Priority

EP 16180774.822 Jul 2016

Published

6 Jun 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C07K 14/38
From fungi: from Aspergillus
C12N 1/14
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 15/80
Vectors or expression systems specially adapted for eukaryotic hosts: for fungi
C12P 21/02
Preparation of peptides or proteins: having a known sequence of two or more amino acids, e.g. glutathione

Improved Filamentous Fungal Host

Life of this patent

Improved Filamentous Fungal Host

Life of this patent

Abstract

Claims17