Purification of virus like particles
AssigneeTakeda Vaccines, Inc.
InventorRoss TAYLOR
Life of this patent
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Abstract
Methods of purifying virus-like particles (VLPs) that are substantially free of process contaminants and infectious agents. The methods incorporate, for example, low-pH treatment during harvest and/or inactivation by a solvent and/or detergent during VLP capture.
Claims37
A method for purifying virus like particles (VLPs), comprising:
- generating or obtaining a cell lysate, culture supernatant, or filtrate containing said VLPs;
- adjusting the pH of the lysate, supernatant, or filtrate to a pH value less than about 5 to generate a pH-adjusted solution;
- and
- removing non-VLP particulates/aggregates from the pH-adjusted solution to generate a purified solution comprising the VLPs.
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A solution comprising a purified population of VLPs, wherein said solution contains at least 50% VLPs with a full length VP1 subunit.
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A method of clearing and/or inactivating live virus in a solution containing the live virus and further containing VLPs generated by the live virus, the method comprising adjusting the pH of the solution containing the live virus to a pH value less than about 5 to generate a pH adjusted solution.
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Dependencies are inferred from the claim text.