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AssigneeThe Research Foundation for The State University of New York
InventorsBruce Futcher, Justin Gardin, Steven Skiena, Alisa Yurovsky, Eckard Wimmer, Steffen Mueller
Estimating the term…
This invention provides a modified protein encoding sequence containing nucleotide substitutions at multiple locations in the protein encoding sequence, wherein the substitutions introduce rare hexamers. These hexamers may be Frame Dependent, or depleted in only the reading frame, or Frame Independent, or depleted in all three frames. Modified protein encoding se quences of the present invention may include modified viruses useful for vaccines.
A modified protein encoding sequence comprising a polynucleotide sequence derived from a target protein encoding sequence, wherein the modified protein encoding sequence encodes a polypeptide having substantially the same amino acid sequence as the polypeptide encoded by the target protein encoding sequence and comprises a plurality of additional hexamers selected from one or more of the group consisting of SEQ ID NO:19 to SEQ ID NO:418 compared to the target protein encoding sequence.
5-9. (canceled)
11-12. (canceled)
A method for reducing the expression of a target protein comprising introducing into the target protein encoding sequence a plurality of hexamers selected from one or more of the group consisting of SEQ ID NO:
(canceled)
A modified protein encoding sequence comprising a polynucleotide sequence derived from a target protein encoding sequence, wherein the modified protein encoding sequence encodes a polypeptide having substantially the same amino acid sequence as the polypeptide encoded by the target protein encoding sequence and comprises at least one of:
26-28. (canceled)
30-35. (canceled)
Dependencies are inferred from the claim text.